/ DoerLBH / Random


2000-01-01 posted in []

here is a test Xinxin is lovely DNA computing? Seelig Nature Multiplex CRISPR computing? GMC Nature fitness evolution, ucsf, kortemme lab David report first Felix polymer into protein and also chromatin what a day rDNA still worth pursuing but have to say more molecular and structural exploration would be much more interesting~ should not use random chosen states, but use the probablities calculated based on all thress sates chromatin looping? information into a review type thing or it could be nothing how to connect my dynamics rigid-body to DNA or protein looping http://www.sciencemag.org/site/feature/data/prizes/ge/2010/fullwood.xhtml another day of entering github journal… MathBio PhD or SynBio or SysBio or CompBio??? Or Google Genomics? FZ talk? Not gonna stop Need to solidify some programs to apply weekend plan decide programs, send refer help contact FY, sned email to several prf visa intel internship sep AZ, CO, CA .. HPC as a fundamental capacity HPC needs innovation: Efficiency, Reliability, Paralleism Programmable Solutions Group FPGA IPG (Intel Processor Graphics) CPU Cores Markov Chain in other axis??? like in environment Can we infer the entire enviorment based on a point In a chessboard, how many grids do we need to mark down entire chess board. stochastic gene expression on JP paper, we can do stochastic modeling thx jai & reply qi for stochastic process of iR is there any better quantificaiton iPSCs basals to think about in a simutaneous form network transsion 7~8 hq, 8-11 422, 11-12 1em, 0-4 373, 4-5 1em, 5-7 531 bayesian in 3D, 7-8 1em, 8-10 mitapp… , catch up in ML, CV, AI, Stoch filter projects outline labs outline school ranks end resort ML AI projects and potential collaborations book flights, compare schools what do I wish to focus make plan for courses next adv physics, app math, sys bio plan time what makes a good biologidt today I am learning Python. A new language that I used to think it is easy and not worthwhile. It turned out to be a huge mistake. Now I find it a very interesting programming tool that can enable me to do many things in data science and a geeky lifestyle. I am very looking forward to it.! review bicohem back to bk lecture PTM Working with proteins detecting protein by UV absorbance spectrophotometer copper-based assays dye-based assays protein purification salting out dialysis centrifuge chromatography with UV detector and fraction collector FPLC / HPLC gel filtration ion exchange chromatography affinity chromatography recombinant tags for affinity chromatography e.g. GST, MBP, His, Strep Rasmussen te al Nature 2011 Nobel protein electrophoresis (SDS, Native, IEF) gel matrix SDS PAGE silver staining 100 times more than blue staining IsoElectric Focusing (IEF) protein identifacation 2D PAGE = IEF + SDS PAGE Western Blotting one protein detection method sandwich chamber amino acid analysis hydrolyzed sample? N-terminal sequencing Edman’s Method protein cleavage chemical cleavage enzymatic cleavage protein 3D structure determination X-ray crystallography homology modeling SAXS SANS NMR spectroscopy EM